Tóm tắt nội dung:

Purified protease from Aspergillus oryzae was immobilized by comlex polymer microencapsulation. Immobilization of enzyme was effected by alginate concentration, type of polymer such as chitosan, xanthan gum, maltodextrin and oncentration of polymer. The properties of immobilized enzyme have been investigated such as percentage of yield, percentage of microencapsulation efficiency, bead size, percentage of leakage of encapsulated enzyme and percentage of encapsulated enzyme retained over 10-week storage at 0 – 40C. Some results are following: optimum concentration of immobilization is 2,0% alginate, alginate/chitosan comlex, and 0,4% chitosan which a immobilized yield, icroencapsulation efficiency and beads size is 78,07%; 73,06% and 1,94mm, respectively. Protease leakage was only 6,65% over 120minutes when encapsulated protease was soaked in de-ionized water while 81,42% retention of enzyme activity was noted in protease capsules over 10 – week storage at 0 – 40C. These results indicated that immobilized enzyme in alginate/chitosan can use for applying on continous process which can make lower price of manufacture.