TÓM TẮT NỘI DUNG:

White spot syndrome virus (WSSV) is an serious viral pathogen responsible for severely economic loss to shrimp farmers. Mortality rate can be up to 100% within 7 – 10 days after infection. Therefore, there should be a procedure for early diagnosing of WSSV on shrimp seed and mature shrimp. The shrimp samples suspected of being infected with WSSV were collected in Cai Nuoc and Dam Doi districts of Ca Mau province. The protocol for WSSV detectiong on collected shrimp samples was carried out by nested PCR. The sequencing results showed that nested PCR procedure surveyed in this study was able to detect accurately WSSV in shrimp samples suspected of being infected with WSSV collected. This procedure had the sensitivity of 10 3 copies and the high specificity with WSSV. This protocol will be used to detect WSSV on shirmp.