TÓM TẮT NỘI DUNG:

Streptococcus iniae is a recognised pathogen of hemorrhage impacting aquaculture production of Asean seabass Lates calcarifer (Bloch, 1790). However, it is difficult when analyses of bacterial antigens and the immune response after vaccination because S. iniae is a Gram-positive bacteria and the cell wall had to be degraded before processing for SDS-PAGE. Different methods for degradation the cell wall of S. iniae isolates to perform protein profiles by using SDS-PAGE were evaluated. Characterize isolates of S. iniae from Lates calcarifer perform the degradation processes included treatment with acetone, lysine, mutanolysine as well as sonication. Each of the degradation process was performed separately or in combinations of sonication. Among the methods had been tested, degradation process by acetone, lysine, sonication or combination showed unclearly band or no band on the electrophoresis gel. Meanwhile, using mutanolysine performed best result with fully detected protein profile as clearly bands between 10 to 100 kDa.